Pathogen reduction in poultry products is a priority of the poultry industry. New performance standards proposed by the U.S. Department of Agriculture’s Food Safety and Inspection Service (USDA/FSIS) aimed at reducing Salmonella and Campylobacter make the challenge more stringent.
The goal of this project has been to develop a rapid, multiplexed, and disposable field-usable device utilizing the combined lateral flow and interdigitated microelectrode (IDE) array as a single transducer for foodborne pathogen detection. Researchers fabricated an IDE array using the standard microfabrication technology and a covalently linked polyclonal antibody specific to Salmonella applied to the surface of the IDE. A high-flow membrane was added to the IDE for liquid application. The team successfully demonstrated the ability to use lateral flow to deliver a fluid sample containing 108 cfu/ml of Salmonella over the electrode array. In addition, the team confirmed that the bacteria cells were binding to the top of the electrodes using a scanning electron microscope and a confocal microscope.
Researchers are currently working on optimizing the electrode surface morphology, antibody surface concentration, and ionic strength of the buffer to improve sensitivity and lower the limit of detection. In an electrode array, multiplexing could be provided by immobilizing a different antibody to each electrode.
Project Contact: Jie Xu